Geronimo the alpaca has been taken from the farm where he lives by government vets supported by a police escort.
Avon and Somerset police officers attended the site in Wickwar, South Gloucestershire, shortly before 11am on Tuesday alongside three people dressed in blue overalls, masks and goggles.
Geronimo has twice tested positive for bovine tuberculosis and a destruction warrant has been ordered for the animal, although the owner, Helen Macdonald, believed the tests returned false positives.
After about 20 minutes the black alpaca was rounded up as other alpacas watched on from a nearby field, before being loaded into a trailer, which then left the scene.
The force later confirmed it was supporting the Animal and Plant Health Agency in executing a court warrant.
A spokesperson for Avon and Somerset police said: “We can confirm officers are in attendance at a farm in the Wickwar area of South Gloucestershire this morning to support the Animal and Plant Health Agency, who are executing a court warrant.
“We’ll always support our partner agencies to carry out their lawful duties and our role is to prevent a breach of the peace and to ensure public safety is protected.”
One woman was briefly arrested after spraying officers with a water pistol but was quickly de-arrested.
The destruction warrant is valid until 4 September and Macdonald wants the Department for Environment, Food and Rural Affairs to allow Geronimo to be tested for a third time or let him live to aid research into the disease.
Soon after police arrived, Macdonald appealed for another meeting with the environment secretary, George Eustice, agregando: “Please don’t execute Geronimo.”